plasmid pbluescript sk(2 (Agilent technologies)
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plasmid pbluescript sk(2
Plasmid Pbluescript Sk(2, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+sk+(2)+plasmid/10__1645_slash_ge___477r__1-91-22-25
Average 90 stars, based on 1 article reviews
Plasmid Pbluescript Sk(2, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+sk+(2)+plasmid/10__1645_slash_ge___477r__1-91-22-25
Average 90 stars, based on 1 article reviews
plasmid pbluescript sk(2 - by Bioz Stars,
2026-09
90/100 stars
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other:Article Title: Involvement of Grb2 Adaptor Protein in Nucleophosmin-Anaplastic Lymphoma Kinase (NPM-ALK)-mediated Signaling and Anaplastic Large Cell Lymphoma Growth Article Snippet: The amount of DNA for each transfection was kept constant at 2 μg by adding an appropriate amount of Article Title: KNAT3/4/5-like class 2 KNOX transcription factors are involved in Medicago truncatula symbiotic nodule organ development. Article Snippet: Plasmid construction For the MtKNOX3 overexpression construct, the MtKNOX3 cDNA sequence was released from the pBlueScript Amp SK (+) vector (Stratagene, La Jolla, CA, USA) using XbaI and KpnI restriction sites and cloned into the binary vector pMF2 (Merchan et al., 2007) under the control of the 35S-CaMV promoter. Article Title: CLONING OF A cDNA ENCODING A 66-kDa Ca 2 +-DEPENDENT PROTEIN KINASE (CDPK) FROM DUNALIELLA TERTIOLECTA (CHLOROPHYTA). Article Snippet: A cDNA clone encoding a Ca 2 1 -dependent protein kinase (DtCPK1) with a calculated molecular mass of 65,746 Da was isolated by sequential immunoand hybridization-screening from a cDNA library of the halotolerant green alga, Dunaliella tertiolecta Butcher (Chlorophyceae).. Primary structure analysis of DtCPK1 revealed a long variable domain preceding a catalytic domain, an autoinhibitory junction domain, and a C-terminal calmodulin-like domain containing 4 EFhand motifs.. Database searches showed that DtCPK1 has a high similarity to CCK1 , a CDPK from the green alga, Chlamydomonas eugamentos Moewus . Article Title: Involvement of Grb2 Adaptor Protein in Nucleophosmin-Anaplastic Lymphoma Kinase (NPM-ALK)-mediated Signaling and Anaplastic Large Cell Lymphoma Growth Article Snippet: The amount of DNA for each transfection was kept constant at 2 g by adding an appropriate amount of Clone Assay:Article Title: Recombinational cloning of the antibiotic biosynthetic gene clusters in linear plasmid SCP1 of Streptomyces coelicolor A3(2). Article Snippet: DH5α F- deoR recA1 endA1 hsdR17(rk- mk+) phoA supE44 λ- thi-1 gyrA96 relA1 Life Technologies, Inc .. ET12567 (pUZ8002) dam dcm hsdM cm km Kieser et al., Article Title: An Integrated Chromosome Map of Microsatellite Markers and Inversion Breakpoints for an Asian Malaria Mosquito, Anopheles stephensi Article Snippet: .. Second, 4 microsatellites were ligated and cloned as a cluster of 2 or 3 repeats in the head-to-tail orientation in the Polymerase Chain Reaction:Article Title: Recombinational cloning of the antibiotic biosynthetic gene clusters in linear plasmid SCP1 of Streptomyces coelicolor A3(2). Article Snippet: DH5α F- deoR recA1 endA1 hsdR17(rk- mk+) phoA supE44 λ- thi-1 gyrA96 relA1 Life Technologies, Inc .. ET12567 (pUZ8002) dam dcm hsdM cm km Kieser et al., Over Expression:Article Title: Identification of Multiple Substrates of the StkP Ser/Thr Protein Kinase in Streptococcus pneumoniae Article Snippet: .. E. coli BL21(DE3) was used as a host for protein overexpression. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Other designation Description Source or reference E. coli strains JM109 endA 1 recA 1 gyrA 96 thi hsdR 17 (r K − m K + ) relA 1 supE 44 Δ( lac-proAB ) [F′ traD 36 proAB lacI q Z ΔM15] Promega BL21(DE3) F − ompT hsdS B (r B − m B − ) gal dcm (DE3) Novagen S. pneumoniae strains Cp1015 Sp1 Rx derivative, str1 hexA 34 Cp1015Δ stkP Sp10 Cp1015 Δ stkP ; Cm r 35 Cp1015 stkPK42R Sp19 Cp1015, stkP ( K42R )-His tag, Cm r , allelic exchange mutant This study Cp1015 stkP-KDtm ( stkP Δ 372 - 659 ) Sp25 Cp1015, stkP :: kdtm -His tag, Cm r , allelic exchange mutant 37 D39 Sp6 Serotype 2, Plasmid Preparation:Article Title: Identification of Multiple Substrates of the StkP Ser/Thr Protein Kinase in Streptococcus pneumoniae Article Snippet: .. E. coli BL21(DE3) was used as a host for protein overexpression. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Other designation Description Source or reference E. coli strains JM109 endA 1 recA 1 gyrA 96 thi hsdR 17 (r K − m K + ) relA 1 supE 44 Δ( lac-proAB ) [F′ traD 36 proAB lacI q Z ΔM15] Promega BL21(DE3) F − ompT hsdS B (r B − m B − ) gal dcm (DE3) Novagen S. pneumoniae strains Cp1015 Sp1 Rx derivative, str1 hexA 34 Cp1015Δ stkP Sp10 Cp1015 Δ stkP ; Cm r 35 Cp1015 stkPK42R Sp19 Cp1015, stkP ( K42R )-His tag, Cm r , allelic exchange mutant This study Cp1015 stkP-KDtm ( stkP Δ 372 - 659 ) Sp25 Cp1015, stkP :: kdtm -His tag, Cm r , allelic exchange mutant 37 D39 Sp6 Serotype 2, Article Title: Contribution to the molecular taxonomy of cultivated populations of mastic trees on Chios Island Article Snippet: .. Gel-extracted amplicons were ligated to Article Title: An Integrated Chromosome Map of Microsatellite Markers and Inversion Breakpoints for an Asian Malaria Mosquito, Anopheles stephensi Article Snippet: .. Second, 4 microsatellites were ligated and cloned as a cluster of 2 or 3 repeats in the head-to-tail orientation in the Mutagenesis:Article Title: Identification of Multiple Substrates of the StkP Ser/Thr Protein Kinase in Streptococcus pneumoniae Article Snippet: .. E. coli BL21(DE3) was used as a host for protein overexpression. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Other designation Description Source or reference E. coli strains JM109 endA 1 recA 1 gyrA 96 thi hsdR 17 (r K − m K + ) relA 1 supE 44 Δ( lac-proAB ) [F′ traD 36 proAB lacI q Z ΔM15] Promega BL21(DE3) F − ompT hsdS B (r B − m B − ) gal dcm (DE3) Novagen S. pneumoniae strains Cp1015 Sp1 Rx derivative, str1 hexA 34 Cp1015Δ stkP Sp10 Cp1015 Δ stkP ; Cm r 35 Cp1015 stkPK42R Sp19 Cp1015, stkP ( K42R )-His tag, Cm r , allelic exchange mutant This study Cp1015 stkP-KDtm ( stkP Δ 372 - 659 ) Sp25 Cp1015, stkP :: kdtm -His tag, Cm r , allelic exchange mutant 37 D39 Sp6 Serotype 2, Expressing:Article Title: Identification of Multiple Substrates of the StkP Ser/Thr Protein Kinase in Streptococcus pneumoniae Article Snippet: .. E. coli BL21(DE3) was used as a host for protein overexpression. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Other designation Description Source or reference E. coli strains JM109 endA 1 recA 1 gyrA 96 thi hsdR 17 (r K − m K + ) relA 1 supE 44 Δ( lac-proAB ) [F′ traD 36 proAB lacI q Z ΔM15] Promega BL21(DE3) F − ompT hsdS B (r B − m B − ) gal dcm (DE3) Novagen S. pneumoniae strains Cp1015 Sp1 Rx derivative, str1 hexA 34 Cp1015Δ stkP Sp10 Cp1015 Δ stkP ; Cm r 35 Cp1015 stkPK42R Sp19 Cp1015, stkP ( K42R )-His tag, Cm r , allelic exchange mutant This study Cp1015 stkP-KDtm ( stkP Δ 372 - 659 ) Sp25 Cp1015, stkP :: kdtm -His tag, Cm r , allelic exchange mutant 37 D39 Sp6 Serotype 2, Cell Fractionation:Article Title: Identification of Multiple Substrates of the StkP Ser/Thr Protein Kinase in Streptococcus pneumoniae Article Snippet: .. E. coli BL21(DE3) was used as a host for protein overexpression. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Other designation Description Source or reference E. coli strains JM109 endA 1 recA 1 gyrA 96 thi hsdR 17 (r K − m K + ) relA 1 supE 44 Δ( lac-proAB ) [F′ traD 36 proAB lacI q Z ΔM15] Promega BL21(DE3) F − ompT hsdS B (r B − m B − ) gal dcm (DE3) Novagen S. pneumoniae strains Cp1015 Sp1 Rx derivative, str1 hexA 34 Cp1015Δ stkP Sp10 Cp1015 Δ stkP ; Cm r 35 Cp1015 stkPK42R Sp19 Cp1015, stkP ( K42R )-His tag, Cm r , allelic exchange mutant This study Cp1015 stkP-KDtm ( stkP Δ 372 - 659 ) Sp25 Cp1015, stkP :: kdtm -His tag, Cm r , allelic exchange mutant 37 D39 Sp6 Serotype 2, |